pierce bca protein assay compatibility
The Pierce BCA Protein Assay is one of the most commonly used protein quantitation assays, so we have determined the compatibility of this assay with each of the lysis buffers or the reagents containing a lytic agent used in Promega reporter assays. All of the lysis buffers and reagents were tested at various dilutions, ranging from 1X to 0.001X.
(e.g., Pierce microplate BCA protein assays). The Pierce microplate BCA protein assay kits and the Invitrogen™ Qubit™ Protein BR Assay Kit were used with a microplate and PCR tube, respectively. Notes and symbols The concentration listed refers to the actual concentration in the protein sample. An Ø denotes compounds that
Features of the BCA Protein Assay Kit—Reducing Agent Compatible include: • Compatibility —assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP • BCA technology —only a slight modification of the standard BCA Protein Assay protocol (15-min incubation with Compatibility Reagent); no precipitation steps required
Features of the BCA Protein Assay Kit include: • Colorimetric —estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm • Uniformity —exhibits less protein-to-protein variation than dye-binding methods (Bradford) • Compatibility —unaffected by typical concentrations of most ionic and non-ionic detergents
• The Pierce BCA Protein Assay Kit − Reducing Agent Compatible is compatible with protein samples containing up to 5mM DTT, 35mM 2-mercaptoethanol or 10mM TCEP. • Certain substances interfere with the Pierce BCA Assay Kit − Reducing Agent Compatible, including chelating agents and strong acids or bases.
The Thermo Scientific ™ Pierce BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the
Scientific Pierce* Protein Assays. Results of these compatibility experiments are summarized in tabular form on the following two pages. The table serves as a guide for assessing which protein assay(s) might be most effective with a given sample type. Use this information as a guide only.
This reducing agent-compatible version of Thermo Scientific's Pierce BCA Protein Assay enables one to measure protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME). Compatible—assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP
compatible with up to 5mm dtt, 35mm 2-mercaptoethanol or 10mm tcep no protein precipitation involved sample volume only 9µl (microplate protocol) compatible with most detergents significantly less (14-23%) protein:protein variation than bradford-based methods allows the use of the superior bca assay in situations in which it is normally unable to …
We did the Pierce BCA assay and ran the standard curve (and were able to estimate protein concentrations in our samples). We had excess standards so we decided to save them and are currently ...
The Compatibility Reagent does not react with BCA protein assay reagents itself. To accommodate the pre-treatment of samples containing DTT, B-ME or TCEP, the BCA protein assay has been adapted for 1.5 ml microcentrifuge tubes. The method requires only 25 μl protein sample and is more convenient than the standard BCA test tube protocol.
Thermo Scientific™ Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard. Manufacturer: Thermo Scientific™ 22663 Catalog No. PI22663 $133.80 / Each Qty Check Availability
Thermo Scientific™ Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants. Manufacturer: Thermo Scientific™ 23250 Catalog No. PI23250 $312.00 / Each Qty Check Availability Add to cart Description
No, hemoglobin is a conjugated protein. It consists of a basic protein globin joined to a non-protein group heme. Hemoglobin will interfere in the Pierce BCA Protein Assay as hemoglobin will absorb...
The BCA™ Protein Assay Kit−Reducing Agent Compatible enables quantitation of total protein in samples while minimizing interference from disulfide reducing agents. The BCA™ Assay is based on the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium and the highly sensitive and selective colorimetric detection of the ...
The BCA Protein Assay Kit from Pierce provides the list of chemicals that have compatibility with this assay and provide an easy methodology how to interpret the results of the assay. We found that the helpful point by means of using the BCA Protein Assay Kit 'Pierce' "is the compatibility with more buffers/salts solvents, and detergents ...
The Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.
P-PER Extracts can be quantified using the Pierce BCA Protein Assay Kit, Reducing Agent Compatible (Product # 23250). T-PER Reagent 78510, 500 ml Heart, liver, kidney and brain Yes Luciferase, β-Gal, CAT, kinase assays, Western blot, ELISAs, immobilized glutathione Pierce BCA Assay (dilute 1:1), Coomassie Plus Assay and Pierce 660 nm Assay
Protein assay reagents involve either protein-dye binding chemistry (coomassie/Bradford) or protein-copper chelation chemistry. Pierce offers seven colorimetric assays for detection and quantitation of total protein. They are all well-characterized, robust assays that provide consistent, reliable results.
Compatible - assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP BCA technology - only a slight modification of the standard BCA Protein Assay protocol (15 minute incubation with Compatibility Reagent); no precipitation steps required Small samples - requires only 25 µl (standard kit) or less than 10 µl (microplate kit) of sample
Thermo Scientific™ Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants. Manufacturer: Thermo Scientific™ 0023250 Catalog No. PI23250 $460.14 / Each Quantity Add to cart Description Specifications
Delivery information: BCA Protein Assay Kit - RAC contains sufficient reagents for 250 tube assays with 25 µl samples; BCA Reagent A, 250 ml; BCA Reagent B, 25 ml; Compatibility Reagent, 10×20 mg; Reconstitution Buffer, 15 ml; Albumin Standard Ampoules, 2 mg/ml, 10×1 ml. Microplate BCA Protein Assay Kit - RAC contains sufficient reagents for 980 microplate assays with 9 µl samples; BCA ...
Thermo Scientific Pierce Protein Assay Test Compound 660 nm BCA Micro BCA †† Microplate BCA-RAC Coomassie Plus Coomassie Modified Lowry n-Acetylglucosamine 100mM 10mM Ø Ø 100mM 100mM n/a Na acetate pH 4.8 100mM 200mM 200mM Ø 180mM 180mM 200mM Na azide 0.125% 0.2% 0.20% 0.01% 0.5% 0.5% 0.2% Na bicarbonate 100mM 100mM 100mM Ø 100mM 100mM ...
Selection of the protein assay When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. The choice among available protein assays is usually based on the compatibility of the protein assay method with the samples.
BCA Reagent B (blue) (contains 4% CuSO 4) Bradford Assay (Pierce Cat. #23236 for 1L, includes BSA standards, 2 mg/mL) Coomassie Blue 250 G Solution (containing Coomassie dye, methanol, phosphoric acid, and solubilizing agents in water. Caution: phosphoric acid is a corrosive liquid.) Protein Solutions: 1) Bovine Serum Albumin (BSA) stock solutions, 1.0 mg/mL 2) Unknown BSA Solutions - I, II ...
Bicinchoninic acid is a highly specific chromogenic reagent for Cu (I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.
This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include ...
This BCA Protein Assay Kit is the reducing agent-compatible version of our popular Thermo Scientific Pierce BCA Protein Assay. The kit enables you to measure protein concentration
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