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The Thermo Scientific™  Pierce ™  BCA   Protein   Assay  Kit is a detergent-compatible formulation based on bicinchoninic acid ( BCA ) for the colorimetric detection and quantitation of total  protein . This method combines the well-known reduction of Cu+2 to Cu+1 by  protein  in an alkaline medium... Overview Highlights Typical Response  Curves .  BCA -based  Protein   Assays .  Standard  assay8 Micro assay9,10,11 Microplate format assay12  Assay  of  protein  solutions containing reducing agents13 Quantitation of immobilized protein14  Protein  in... Both a  standard   assay  for concentrated  proteins  and a micro  assay  for dilute  protein  solutions are described below.  BCA  serves the purpose of the Folin reagent in the Lowry  assay , namely to react with complexes between copper ions and peptide bonds to... Selection of the  Protein   Assay  Selection of a  Protein   Standard   Standard  Preparation  Standards  for Total  Protein   Assay  Compatible and Incompatible Substances

Stoscheck (1990) has suggested that the  BCA   assay  will replace the Lowry because it requires a single step, and the color reagent is stable under alkaline conditions. Both a standard  assay  for concentrated  proteins  and a micro  assay  for dilute  protein  solutions are described below. Principle.  BCA  serves the purpose of the Folin reagent in the ... When using the enhanced tube protocol (i.e., incubating at 60°C for 30 minutes), the working range for the  assay  shifts to 5-250 μg/mL, facilitating detection of more dilute samples. Figure 5.  BCA   Protein   Assay  Kit protocol. Rapid Gold  BCA   Protein   Assay  Kit protocol How  does the Pierce  BCA   Protein   Assay  work? The  BCA   Protein   Assay  combines the well-known reduction of Cu2+ to Cu1+ by  protein  in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid ( BCA ). What is the wavelength of  BCA ? 562 nm This applies for responses pr

The  Pierce   BCA   Protein   Assay  is one of the most commonly used  protein  quantitation  assays , so we have determined the  compatibility  of this  assay  with each of the lysis buffers or the reagents containing a lytic agent used in Promega reporter  assays . All of the lysis buffers and reagents were tested at various dilutions, ranging from 1X to 0.001X. (e.g.,  Pierce  microplate  BCA   protein   assays ). The  Pierce  microplate  BCA   protein   assay  kits and the Invitrogen™ Qubit™  Protein  BR  Assay  Kit were used with a microplate and PCR tube, respectively. Notes and symbols The concentration listed refers to the actual concentration in the  protein  sample. An Ø denotes compounds that Features of the  BCA   Protein   Assay  Kit—Reducing Agent Compatible include: •  Compatibility  — assay  samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP •  BCA  technology —only a slight modification of the standard  BCA   Protein   Assay  protocol (15-min incubation with

Protein   Assay   Kits  are Available in Stock. 24/48H Delivery $166.60 / Each Qty Add to cart Description Specifications Safety and Handling Description Thermo Scientific™ Pierce  BCA   Protein   Assay   Kit  remains one of the most popular  protein  quantitation methods worldwide. BCA   Protein   Assay   Kit  ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of  proteins  in solution. The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by  protein (s). BCA   Protein   Assay   kit  provides a colorimetric detection and quantification of total  protein  content even in the presence of detergents, measuring total  protein  concentration of pure  proteins , extracts or lysates. The  Kit  is based on the chelation of bicinchoninic acid ( BCA ) with the cuprous cation (Cu+1), which is generated by reduction of ... Thermo Scientific ™ Pierce ™  BCA   Protein  Assa $186.80 / Pack Catalog No. PI23209 The